Omega-3 & vitamin B12 increased nerve growth factor (boosts acetylcholine) in the brain after 2 generations.

www.ncbi.nlm.nih.gov

Omega-3 and vitamin B12 supplementation in rats increased nerve growth factor (NGF), which helps grow new brain cells and boosts acetylcholine levels and improved cognitive performance after 2 generations in rats.

The omega-3 and B12 also increased brain-derived neurotrophic factor (BDNF) and vascular endothelial growth factor (VEGF) which both also help grow new brain cells and maintain the blood-brain barrier.